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現在位置华东15选5浙江风采网>實驗試劑制品>生化試劑>熒光染料>DiD紅色熒光染料標記細胞膜 貨號22034
DiD紅色熒光染料標記細胞膜 貨號22034
DiD紅色熒光染料標記細胞膜 貨號22034
  • DiD紅色熒光染料標記細胞膜 貨號22034

华东15选5彩乐乐: DiD紅色熒光染料標記細胞膜 貨號22034

华东15选5浙江风采网 www.hdnka.com 產品報價:詢價

更新時間:2019/3/7 10:37:32

地:美國

牌:AAT Bioquest

號: 22034

廠商性質: 生產型,

公司名稱: 西安百螢生物科技有限公司

產品關鍵詞:

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第五曉東 : (18602671603) (029-68064558)

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產品簡介:          

         染料DiI, DiO, DiD 和 DiR是一類親脂性熒光染料家族,用于標記細胞膜和疏水性組織。這是一類環境敏感型熒光染料,當它們與膜結合或者與親脂性生物分子(例如蛋白質,雖然在水中其熒光強度很弱)結合時,其熒光強度顯著增強。它們具有很高的淬滅系數,偏光依賴性和很短的激發壽命。一旦應用于細胞中,這種染料會在細胞內質膜中逐步擴散,導致在其最佳濃度條件下,將整個細胞染色。

         它們不同的熒光顏色:DiI(橙色熒光)、DiO(綠色熒光)、DiD(紅色熒光)、DiR(深紅色熒光),為活細胞多色彩熒光成像分析和流式細胞術提供了一種便捷的工具。DiO和DiI可以分別與標準的FITC和TRITC濾光器一同使用。其中,DiO可以被633 nm He-Ne激光激發,并且具有比DiI更長的激發和發射光波長,為標記細胞和組織的那些本身就具有本底熒光的染料提供了非常卓越的替代品。

        DiR在活體成像或者示蹤中非常有用,因為它們所發射的紅外光可以高效地穿過細胞和組織,并且在紅外光范圍內,其本底熒光水平很低。

英文注釋:

     DiI, DiO, DiD and DiR dyes are a family of lipophilic fluorescent stains for labeling membranes and other hydrophobic structures. The fluorescence of these environment-sensitive dyes is greatly enhanced when incorporated into membranes or bound to lipophilic biomolecules such as proteins although they are weakly fluorescent in water.

      They have high extinction coefficients, polarity-dependent fluorescence and short excited-state lifetimes. Once applied to cells, these dyes diffuse laterally within the cellular plasma membranes, resulting in even staining of the entire cell at their optimal concentrations. The distinct fluorescence colors of DiI (orange fluorescence), DiO (green fluorescence), DiD (red fluorescence) and DiR (deep red fluorescent) provide a convenient tool for multicolor imaging and flow cytometric analysis of live cells. DiO and DiI can be used with standard FITC and TRITC filters respectively.

      Among them DiD is well excited by the 633 nm He朜e laser, and has much longer excitation and emission wavelengths than those of DiI, providing a valuable alternative for labeling cells and tissues that have significant intrinsic fluorescence. DiR might be useful for in vivo imaging or tracing due to the effective transmission of infrared light through cells and tissues and low level of autofluorescence in the infrared range.

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